
H3K79me3 polyclonal antibody
产品名称: H3K79me3 polyclonal antibody
英文名称: H3K79me3 polyclonal antibody
产品编号: PAB14050
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of H3K79me3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 79 (H3K79me3).
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Storage Buffer:
- In serum (0.05% sodium azide)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:500-1:1000)
Dot Blot (1:50000)
Western Blot (1:500)
Immunofluorescence (1:200)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Role for 53BP1 Tudor domain recognition of p53 dimethylated at lysine 382 in DNA damage signaling.
Kachirskaia I, Shi X, Yamaguchi H, Tanoue K, Wen H, Wang EW, Appella E, Gozani O.J Biol Chem. 2008 Dec 12;283(50):34660-6. Epub 2008 Oct 7.
- 2.
- High-resolution profiling of histone methylations in the human genome.
Barski A, Cuddapah S, Cui K, Roh TY, Schones DE, Wang Z, Wei G, Chepelev I, Zhao K.Cell. 2007 May 18;129(4):823-37.
- 3.
- Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.J Biol Chem. 2003 Jun 27;278(26):24217-24. Epub 2003 Apr 15.
- Applications
- ELISA
- To determine the cross reactivity of H3K79me3 polyclonal antibody (Cat # PAB14050) with other histone H3 modifications, a Dot Blot analysis was performed.
Tested histone H3 modifications include mono- and dimethylation of the same lysine and mono-, di- and trimethylation of lysine 9, 27 and 36.
One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane and detected with the antibody diluted 1 : 50,000.
Figure shows a high specificity for the modification of interest.
- Dot Blot
- ELISA was performed using a serial dilution of H3K79me3 polyclonal antibody (Cat # PAB14050) in antigen coated wells.
The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1 : 70,000.