CDC25B (phospho S353) polyclonal antibody
产品名称： CDC25B (phospho S353) polyclonal antibody
英文名称： CDC25B (phospho S353) polyclonal antibody
产品编号： PAB0433
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25B.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S353 of human CDC25B.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Nuclear export signal in CDC25B.
Uchida S, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Yoshida M, Ishizaka Y, Yamashita K.Biochem Biophys Res Commun. 2004 Mar 26;316(1):226-32.
- 2.
- Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A.Int J Mol Med. 2004 Mar;13(3):431-5.
- 3.
- Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Wu W, Slomovitz BM, Celestino J, Chung L, Thornton A, Lu KH.Cancer Res. 2003 Oct 1;63(19):6195-9.
- Applications
- Western Blot (Cell lysate)
- The CDC25B (phospho S353) polyclonal antibody (Cat # PAB0433) is used in Western blot to detect phospho-CDC25B-S353 in HepG2 cell lysate
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC25B (phospho S353) polyclonal antibody (Cat # PAB0433) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
- Entrez GeneID:
- 994
- Protein Accession#:
- NP_068660;P30305
- Gene Name:
- CDC25B
- Gene Alias:
- -
- Gene Description:
- cell division cycle 25 homolog B (S. pombe)
- Omim ID:
- 116949
- Gene Ontology:
- Hyperlink
- Gene Summary:
- CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
- Other Designations:
- OTTHUMP00000030137,OTTHUMP00000030138,OTTHUMP00000030139,cell division cycle 25B
- Gene Pathway