Histone H3 (K9/14ac) polyclonal antibody
产品名称： Histone H3 (K9/14ac) polyclonal antibody
英文名称： Histone H3 (K9/14ac) polyclonal antibody
产品编号： PAB0652
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (acetylated lysines 9 and 14).
- Immunogen:
- A synthetic peptide (conjugated with KLH) containing the acetylated lysines 9 and 14 (or [K9/14ac]) of human histone H3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In PBS (0.1% BSA, 0.01% thimerosal)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- The optimal working dilution should be determined by the end user.
- Note:
- This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells.
Vincent A, Perrais M, Desseyn JL, Aubert JP, Pigny P, Van Seuningen I.Oncogene. 2007 Oct 4;26(45):6566-76. Epub 2007 Apr 30.
- Applications
- ChIP
- ChIP results obtained with the Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652). ChIP assays were performed using U-2 OS cells.
- Immunofluorescence
- Immunofluorescence with the Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652).
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
(A) Cells were immunofluorescently labelled with the Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC.
(B) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained.
- ELISA
- ELISA is a quantitative method used to determine the concentration of a primary antibody using a series of dilutions of crude sera, affinity purified Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652) and flow-through in antigen coated wells.
The antigen in this case is the peptide which was used to raise the antibody.
We plotted the absorbance versus antibody dilution to estimate the TITER : 1 : 18,000 for crude serum and 1 : 150,000 for affinity purified Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652).